But these devices are so new, and so sought-after, any money off them was worth taking advantage of. But we were wrong again.Ģ021 saw savings of up to $100 / £100 on both models, which might seem like a drop in the ocean compared to their overall price. Never did we think that would happen with the 2021 MacBook Pro Apple released just a month before the Black Friday sales. The newly released laptop was the last thing we expected to see a deal on, but we weren't about to argue. The arrival of the new iPad Pros also meant a drop in price on many other models too, including the iPad Air and iPad mini.Īpple MacBook: In 2020 we were really shocked to see Apple's M1 MacBook discounted by up to £107 by some retailers. It's never an exact science, so our advice would be to have a budget in mind, and when you see an Apple Black Friday deal that fits your bill, grab it before it's gone.Īpple Black Friday: What happened in 2021?Īpple iPad: 2021 gave us some amazing Apple Black Friday and Cyber Monday deals, with between £50-£100 off the brand new 2021 iPad line-up. In 2021, the best Apple Black Friday deals were definitely closer to the weekend itself, but many of the biggest discounts came up in the week before. That said, every year we are seeing retailers start their Black Friday sales early, with most offering deals of some kind as early as October. And Cyber Monday will be hot on its heels on 28 November. Apple Black Friday 2022: when is it?īlack Friday is always the Friday immediately after Thanksgiving, which means in 2022, the Apple Black Friday sale will officially kick off on 25 November. For all the best offers, head over to our dedicated Apple deals page. Plus you'll also find some great deals still going strong right now. Obviously there's a while until the main event, so, for now, let's take a look at what we already know about next year's event. And with Apple's track record for product releases, we expect to see more of the same for Apple Black Friday 2022. This deal has since expired and we haven't tracked a discount on the HomePod mini in a few months.The arrival of new products this year also meant there was a number of impressive price cuts on older, but still brilliant, models. One of the year's first notable discounts on the HomePod mini arrived this past July, with B&H Photo discounting the speaker to $89.95, from $99.00. In this guide, you'll find the best currently available price for the HomePod mini at major Apple resellers including Adorama, Amazon, B&H Photo, Best Buy, and MacMall (which is now integrated with TigerDirect). The device is also a home hub for HomeKit devices, allowing users to control their Hue bulbs, Ecobee thermostats, and more using their voice. With HomePod mini, Apple Music subscribers can easily stream their favorite artists, playlists, radio stations, albums, and more by talking to Siri. In the fall of 2021, Apple added three bright new colors to the HomePod mini line: Blue, Yellow, and Orange. This device is a smaller iteration on Apple's in-home speaker, bringing the price down to a more affordable $99 price tag. In the wake of the discontinuation, the HomePod mini is now the focus for Apple. In March 2021, Apple officially discontinued the original HomePod. Apple launched the HomePod in February 2018 with a premium $349.00 price tag that matched the company's marketing angle, which presented the smart speaker as a music-first device with excellent sound quality.
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In a predominantly digital camera market, it’s important the existence and strength of this photography community continues. The journey of film is not a solo journey. “One of our personal favourites is the Olympus ACE-E.”įinding the best film camera? Think of it as a journey And with a mechanical body, they are built to last!” Janie says. The fully manual operation requires you to learn how to properly expose your photos, encouraging you to be totally present when shooting. “Olympus has made some excellent rangefinders in various models with a similar build. Because there isn’t a mirror slapping around inside, rangefinder cameras are more compact, lighter and provide higher image quality as there’s naturally less vibration. There are many benefits to using a rangefinder. An image is focused and composed by looking through the little window cutout at the top left of the camera, rather than through the lens as experienced with an SLR. Be warned, rangefinders may take some getting used to if coming from an SLR kit. Rangefinder cameras often have this blend of manual and auto exposures and are perfect for sinking your teeth a little further into the film photographic process. If you’re an intermediate photographer, any film camera with a blend of manual and auto exposures is going to provide a gradual learning experience for film beginners as shooting manually encourages a forced understanding of the exposure triangle (with a safety net). Address renters can manage their mail through a phone app. USPS sent my box to Bangladesh instead of England. Anytime Mailbox provides mailing addresses mostly in the US for private and business use. Recently I had a transatlantic forwarding request for a box containing several items. Shipping a box containing 15 envelopes and 3 bags of online clothing purchases is $70, avoid doing that if possible. Shipping an envelope containing 1or 2 plastic cards overseas is $7. Each mail item has a unique ID in the digital system which prevents mix-ups or getting lost. Paying $6.98/month for basic service which requires 50-cent charges for every piece of incoming mail, and action requests, with additional charges for shipping. Make sure the forwarding request for all of them is provided, and your linked payment method works, or you can't approve the forwarding request. Took me a few days to get the hang of the app, most complicated thing is when you have to select multiple items of mail to forward them all in the same package. I just find it inconvenient that my mail service is closed intermittently one or two days a week without notice. Helpful and accessible customer service available by phone or email, staff for the app as well as staff for your local mailbox. Freshdesk integrated all of the varied support apps Anytime Mailbox was using into one, and according to Nathalie, they all work together seamlessly.Good and useful service, it's what you make of it. Their support team handles multiple channels, such as phone, email, and chat, which helps unify all customer queries. A unified experienceĪpart from Freshdesk Contact Center, Anytime Mailbox uses other Freshworks products like Freshdesk Support Desk and Freshchat. According to Nathalie, “Freshdesk Contact Center checked all the boxes and continues to provide the best support.” And she loves Freshdesk Contact Center’s customizable, user-friendly interface. Her ideal system would allow her teams to convert phone calls into support tickets. And one of the things which Nathalie specifically needed was an intuitive hands-on approach while, naturally, not being pressured during the evaluation process. In fact, one of her very first projects after joining Anytime Mailbox was to find software that would streamline their customer support functions.Īnytime Mailbox reviewed many different vendors, including, Salesforce, but none had the suite or combinations of products and services they required. This disparate setup created gaps in communication between the systems, and often, things would not sync up as well as they should”. Furthermore, you can find the Troubleshooting Login Issues section which can answer your unresolved problems and equip you with a lot of relevant information. LoginAsk is here to help you access Anytime Mail Login quickly and handle each specific case you encounter. Anytime Mailbox’s VP of Operations, Nathalie Schroeder, said, “It was frustrating using multiple applications, one application for our toll-free number, another for our live chat, and then we’re using emails to respond to customer queries. Anytime Mail Login will sometimes glitch and take you a long time to try different solutions. The struggle of multiple toolsīefore Freshdesk Contact Center, the customer support team utilized several different software tools. To deliver superior customer service, Anytime Mailbox uses Freshdesk Contact Center to handle both inbound and outbound calls for their end user and operator support functions. They are digital nomads and wanderlusters at heart, excited to show up each day and ready to continue enhancing the quality of life for individuals the world over. Headquartered in Southern California (USA) with offices in Manila and Singapore, they are truly a distributed company, employing location-independent teams based around the globe. Use your key to access your private mailbox and grab your mail. Anytime Mailbox Digital Mailbox Management Software improves productivity and time-savings while also providing security and convenience to businesses and individuals alike. Pick up your mail whenever its most convenient for you. Their mission is to transition all postal mail onto smartphones and laptops across the world. Anytime Mailbox is a leading digital mailbox and postal mail management software company. Scavenging and carrying concrete is hard work, but an additional worker eases the burden a little.Ī sturdy sledgehammer is just the right tool for quickly crushing concrete into manageable pieces. Unlocks the scavenging of concrete deposits and production of concrete that is needed in the construction of more advanced buildings. Other societies are still out there and willing to exchange goods once you have built a trade center to coordinate these transactions. Your colony is not the only one in the world. A maintenance depot automatically fixes damaged buildings and cleans contamination within its work area, provided it is staffed and supplied properly.Ī proper relationship between workers means more products created in toolshops and tailors and less thrown at those you're working alongside. List of technologies ĭelegating the repairs in a large colony lets you focus on more important things.
Nevertheless, in 1913, against her mother's objections, 21-year-old Stella Benson left her home in Shropshire for an independent life in London, where she took up a series of jobs to support herself, producing novels which eventually brought her fame as a modernist writer and launching her own "private Stellarian Suffrage Campaign" of argument and persuasion.īy the spring of 1913, the suffrage movement had turned confrontational. Judged too sick to attend school, she had been tutored at home by governesses. Moreover, Benson was a frail child, beset by serious respiratory illnesses, often bedridden for months. At that time, it was usual for girls of her upper-class status to remain in their parents' keep, reared as Edwardian ladies, supported at home until marriage. It is difficult to imagine a less likely candidate for feminine independence than Stella Benson. In 1910, forced by severe illness to spend 18 months convalescing in a sanatorium, Benson, aged 18, devoted her time to reading and reflecting on the feminist ideas and arguments which were then drawing considerable attention in the popular intellectual and political press she determined to strike out on her own. Stella Benson came of age during the woman's suffrage movement in early 20th-century England. I Pose (1915) This Is the End (1917) (poems) Twenty (1918) Living Alone (1919) Kwan-Yin (1922) The Poor Man (1922) Pipers and a Dancer (1924) (self-illustrated essays) The Little World (1925) The Awakening: A Fantasy (1925) Goodbye, Stranger (1926) (self-illustrated essays) Worlds within Worlds (1928) The Man Who Missed the Bus: A Story (1928) The Far-Away Bride (1930, republished as Tobit Transplanted, 1931) Hope Against Hope and Other Stories (1932) Christmas Formula and Other Stories (1931) (with Count Nicolas de Toulouse Lautrec de Savine, K.M.) Pull Devil, Pull Baker (1933) Mundos: An Unfinished Novel (1935) Poems (1935) Collected Short Stories (1936). with husband moved to China with husband, settling in Mengtsz (1922–25), then Shanghai, followed by Lung Ching Tsun (1925–27), Nanning (1929–30), Hong Kong (1930–31), and Pakhoi (1931–33). Benson silver medal for service to literature (1932).īegan lifelong practice of writing diary (1901) wrote I Pose (1915), a feminist satire about the suffrage movement wrote novels, poems, and short stories, mixing fantasy and satire traveled alone, often ill but self-supporting, to Berkeley, California (1918–19), to China and India (1920–21) traveled to the U.S. Awards:įrench Vie Heureuse Prize (1932) for Tobit Transplanted ( published in U.S. Born Stella Benson on January 6, 1892, in Shropshire, England died on December 6, 1933, in Hongay in the Chinese province of Tongking (now Vietnam), of pneumonia and heart failure buried on the Ile de Charbon near Baie d'Along daughter of Caroline Essex (Cholmondeley) and Ralph Beaumont Benson, both landed gentry educated at home married Shaemus (James) O'Gorman Anderson (a Chinese government customs official), on Septemchildren: none. Name variations: Stella Benson O'Gorman Anderson. Modernist English novelist, poet, and travel writer who actively campaigned for women's rights before and during World War I and in Hong Kong during the early 1930s. He went on to serve as director of Morehouse’s School of Religion from 1965 to 1969. In 1957 McCall took over the pastorate at Providence Baptist Church in Atlanta. The storm will break! ” ( Papers 3:118 ). McCall offered his support to King in the early stages of the Montgomery bus boycott, writing, “ Do not despair. Following King’s trial sermon at Dexter, entitled “The Three Dimensions of a Complete Life,” McCall gave a sermon there entitled “The Four Dimensions of a Complete Life.” Dexter awarded its pastorate to King, and McCall wrote to congratulate him.Įarlier in their careers, McCall and King exchanged speaking engagements, with McCall speaking at a Youth Day at Dexter and King speaking at Religious Emphasis Week at Fort Valley State College. McCall and King competed directly for the open Dexter Avenue Baptist Church pastorate in early 1954. “He would sometimes, if necessary, stay up all night to make certain that he got an idea” (McCall, 31 March 1970).Īfter completing his studies at Crozer in 1951, McCall attended Temple University and was dean and chaplain at Georgia’s Fort Valley State College from 1951 to 1957. “He began to take his studies more seriously he began to take preaching more seriously,” he recalled. McCall observed a change in King when he entered the seminary. McCall shared King’s passion for justice: they were part of a group that considered suing a New Jersey tavern owner who had refused to serve them on the basis of race in 1950. Although six years older than King, the two formed a close friendship while at Morehouse. McCall was born in Conway, South Carolina, on 23 August 1923, and served in the Army during World War II. You can listen to every pitch of the 2017 season live on Sports/Talk 960 The Bull or streamed live on the TuneIn Radio app.A Baptist minister and close friend of Martin Luther King’s at both Morehouse College and Crozer Theological Seminary, Walter McCall described his relationship with King as the type of “friends who share in the totality of your life’s experiences.” McCall remembered King as “an ordinary student” who “loved the lighter side of life as any normal boy would do” (McCall, 31 March 1970). The Astros (21-14) will throw right-hander Justin Ferrell (2-2, 7.86) in the second game of three between the clubs. with lefty Wes Benjamin (2-2, 6.93) for the Wood Ducks (13-21). The series continues on Saturday at 6:00 p.m. The best play from the Astros came on a Johnny Sewald diving catch in right to rob a single from Brallan Perez in the eighth. Finally, Ricardo Rodriguez worked a 1-2-3 ninth to keep the game within reach.īoth teams also traded stellar defensive plays throughout the game, including a sliding catch by O'Neill in center, and diving stops by Josh Altmann at first and Morgan at short. Brady Feigl stranded a one-out triple with a groundout and a strikeout in the eighth. The game was in the bullpen's hands in the seventh, when Scott Williams made his Wood Ducks debut with a scoreless frame with a strikeout leaving runners on second and third. The Woodies would put at least one runner on base from innings two through eight. Both teams finished the night 0-for-7 with runners in scoring position. In the bottom half the Woodies got a two-out double from Eduard Pinto but could not bring him home. Martinez allowed a solo home run to Dexture McCall, then a two-out rally with an RBI single from Jason Martin to give the Astros the lead 2-1. However it would be Josh Morgan supplying the run, with a deep sacrifice fly to center field.Ī struggle in the losing streak has been shutdown innings - the inning after scoring a run. Michael O'Neill would walk and La O would steal third during Chuck Moorman 's at bat. Luis La O led off the inning by reaching on a throwing error from Anibal Sierra at shortstop. In the bottom of the fourth, the Woodies would reward Martinez with a run. Martinez retired the side in order in two of the first four innings and did not have a runner a scoring position against him. Emerson Martinez only allowed two runs to the top run-scoring offense in the league, but Down East couldn't stage a comeback to support the quality start. KINSTON, NC - Stellar pitching could not cover up a lack of offense in the Wood Ducks' fifth straight loss, 2-1 to the Buies Creek Astros. Assembly was completed in less than an hour’s. Place this durable rug at the. The white, vinyl lattice screen with planter box attached is easy to move about and position because it is freestanding and light-weight (with plants, pots and growing soil removed). This is the perfect floor covering for lifes softest moments. To capture the lavish essence of your new area rug upon arrival, we recommend to gently fluff the shag rug by using a stiff brush, vacuum safe for high-pile rugs or your hand. The exquisite pattern creates the perfect depth and dimension in your space, allowing you and your loved ones to sit back and relax. It doesnt get more classic than this: a Moroccan-inspired rug. Place this durable rug at the foot of the bed to make your first steps of the day extra luxurious, or anywhere in the home that could use a graceful touch. What are some popular features for White Lattice Vinyl Fencing Some popular features for White Lattice Vinyl Fencing are uv protected, water resistant and painted. This is the perfect floor covering for life's softest moments. The best-rated product in White Lattice Vinyl Fencing is the Ashton 3.7 ft. Normally ships out within 3-4 business days.It doesn't get more classic than this: a Moroccan-inspired rug. 1/8-in x 48-in x 8-ft White Vinyl Traditional Lattice. From 77.99 84.18 (898) Rated 4.5 out of 5 stars.898 total votes. White Plastic Lattice Fence (48 In.) for Use with Fanta-Sea Pools Single Pack. 3/4-in x 24-in x 6-ft White Plastic Traditional Decorative Screen Panel. We offer Free Fed Ex Shipping TO MOST locations in the 48 states. Showing results for 'white lattice panel' 181,723 Results. Buy White Plastic Lattice Fence (48 In.) for Use with Fanta-Sea Pools Single Pack: Decorative Fences - FREE DELIVERY possible on eligible. Where to use them.they really can go anyplace. If you can’t find the product you want in the search results for White Lattice, you can try. We carry millions of home products with free shipping from furniture and decor to lighting and renovation. We give you a quality product at a fabulous price. You have searched for White Lattice and this page displays the best product matches we have for White Lattice to buy online in September 2022. We put the drawer & door handles on the inside of the cabinet so they ship safely. We recommend them.Ĭonstruction.Our Single Lattice Wicker Cabinet is made with tried & true methods of furniture making. First we frame out our cabinets with good size pieces of mahogany wood. Then different textures of wicker are wrapped & hand woven over the frames. Our frames are stronger & we weave thicker textures of wicker than you normally see. Look at the fronts doors. Please notice the better texture of wicker lattice work on our door fronts. They also prevent the dust from accumulating in the wicker weave on top. Note: This cabinet may be installed horizontally or vertically. Glass Top Available $35.00.Glass Tops provide a flat surface and protect the top from being scratched or chipped. Cabinet will hold up to 11 bottles 3-3/4 in diameter. 34 " 1/2 also Available in Caramel, Teawash and White Wash. SpamSweep will only filter one account in trial mode. SpamSweep 1.6.2 is priced at $25.00 (USD) for a single-user license. This version adds Snow Leopard compatibility, improves accuracy, and includes various bug fixes. Apple Mail, Eudora, Entourage, Postbox, PocoMail, Netscape, Spicebird, Claws Mail, Opera, PowerMail, Sylpheed, Evolution, SeaMonkey, Mulberry, GyazMail. (I would have included MailForge 3. Since spam messages are deleted on server, the user can keep their device spam-free by simply leaving their Mac on at home. Well, Ive been running Gyazmail and Eudora 6.2.4 (and Thunderbird 13.0.1) in parallel for a while now. Finally I can give GyazMail the full 5 stars Well, spam filtering was the only. Apple Mail, Eudora, Entourage, Postbox, Netscape, Spicebird, PocoMail, Opera, PowerMail, Claws Mail, Evolution, SeaMonkey, Sylpheed, Mulberry, GyazMail. SpamSweep is also uniquely positioned to filter mail for an iPhone or other mobile device. GyazMail has reached perfection artspraken. Reading some reviews of eM Client, I was intrigued. Im an old school email client addicted, once, to the Qualcomm Eudora client, and after it was discontinued, an orphan, never satisfied by the alternatives offered by the market. There's no need to install plugins or manually configure rule filters. GyazMail is an e-mail client that is developed from scratch to be a simple, easy-to-use e-mail client with many features, based on the Cocoa framework. Sign up to receive home sales alerts in Los Angeles Street in Los Angeles, CA. Explore 25+ apps like GyazMail, all suggested and ranked by the AlternativeTo user community. Account settings are imported automatically and the user's email client is triggered whenever new good mail is available. The application currently integrates seamlessly with Apple Mail, Microsoft Entourage, GyazMail, Mailsmith, and Eudora. SpamSweep seamlessly combines many filtering technologies, including domain and relay blacklists, sender whitelisting, and a bayesian filter to automatically delete spam messages before they're downloaded by the user's email client. SpamSweep is an advanced bayesian spam filter with a simple, easy to understand interface. Bains Software has announced the immediate availability of SpamSweep v1.6.2, an update to its powerful spam filter for Mac OS X. GyazMail is an e-mail client that is developed from scratch to be a simple, easy-to-use e-mail client with many features, based on the Cocoa framework. It is a relaxing one to make because they are not m. She specializes in decorative floral creations for weddings and other special events. It is a traditional Japanese origami design, suitable for a beginner. Hiromi Yamazaki teaches paper flower-making classes at the Boule De Neige floral paper art shop in Tokyo. Japanese Paper Flowers has all the information and all of the necessary patterns you need! With just some scissors, a few sheets of colorful tant origami paper, and a few drops of crafts glue-all of which can be found online and in paper stores-you can create breathtaking paper flowers in no time at all! Perhaps best of all, there are instructions for exquisite wearable pieces, sure to draw appreciative comments and perfect as gifts for loved ones: Fold each corner into the middle crease to make what is known in origami as a blintz base. Make sure to press hard on the crease lines so they're easy to see once the paper has been unfolded. There are instructions showing how the designs can be used in 18 amazing ways-for decorating, selling, gift giving and entertaining-including: Unfold, then fold the paper in half vertically. Simple instructions, templates and colorful step-by-step photos show even novice paper crafters how to create each project, and how to gather them into incredible paper flower bouquets. Kirigami is the art of cutting paper, although as far as I know, nowadays these paper flowers would be considered paper craft. Festive poinsettias-blooms that will look fresh throughout the entire holiday season.CHIPBOARD Simple one piece fold-and-tuck construction. 3 bold sunflowers-in slightly different shapes and sizes, these cheerful flowers create a sunny bouquet when grouped together From backyard flower beds to vegetable and herb gardens, check out our best gardening ideas.Delicate cherry blossoms-enjoy the wonders of cherry blossom season in your own home.Along the way, take the time to flatten the folds so they are crisp. Unfold the paper and you get yourself a square These tulips are one of the easiest origami flowers you can make, so it’s perfect for kids who are new to paper folding. Colorful tulips-carry a piece of spring with you throughout the entire year Fold all of these corners to the middle for the last time. Simply fold the top right corner to the left edge and then cut out the excess paper on the bottom.Frilly carnations-full of intricate folds and textures, these popular flowers are just as much fun in paper form.5 different roses-these romantic flowers can be mixed and matched to create a natural looking bouquet.The sculptural quality of these flowers is an amazing and creative way to add a touch of beauty to your home, or to create low-cost decorations for a wedding, a baby shower, or any other special event.Īll of the most popular blossoms are represented here, including: This inspirational how-to guide shows you how to make 31 gorgeous designs that can virtually pass for the real thing-except they will never wilt! Japanese kirigami (cut paper) flowers are delicate and beautiful examples of paper craft, but are surprisingly easy to make. With Eve’s agreement, this tutorial is my updated and revised version of the kusudama tutorials she originally posted there.Learn how to bring a Japanese aesthetic into your life with these elegant works of floral art. Easy japanese origami Japanese Traditional Art. (You may also recognise the Kusudama flower as the logo for the now-defunct papercraft site, Folding Trees, that I used to run with Eve Henley. Origami japanese origami Origami: The Art of Paper Folding japanese origami How to make an Origami Flower. Glue 12 flowers together into a full kusudama ball to make a beautiful gift or stunning decor for parties and weddings.Add floral wire stems to the bottom of each flower and assemble them into a paper bouquet.Make a single kusudama flower, as shown here, as a lovely place setting, wedding favour, or buttonhole.A decorative paper version can be created by first folding origami flower petals and gluing them together into flowers, and then assembling 12 flowers into a decorative ball.īut these lovely paper flowers are so versatile and have many uses in addition to assembly into a kusudama ball: Japanese Kusudama balls are traditionally assembled from real flowers and herbs, and used as incense or pot pourri. They make great gifts for Valentines Day, Mothers Day, Fathers Day, birthdays, etc. Analysis of the completed genomes of Homo sapiens, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, and a red alga (Cyanidioschyzon merolae 10D) did not reveal the presence of a KCBP. A calmodulin-binding kinesin called kinesin-C (SpKinC) isolated from sea urchin (Strongylocentrotus purpuratus) is the only reported kinesin with a CBD. The MyTH4 and talin-like domains (found in some myosins) are not found in other reported kinesins. Terms of Use.Kinesin-like calmodulin-binding protein (KCBP), a member of the Kinesin-14 family, is a C-terminal microtubule motor with three unique domains including a myosin tail homology region 4 (MyTH4), a talin-like domain, and a calmodulin-binding domain (CBD). The results of each algorithm are displayed on an interactive graphical display that lets you zoom in all the way to the residue level if required and lets you select potential open reading frames to translate by clicking on start codons or on entire open reading frames.Ĭopyright © 2022 MacVector, Inc. It includes a simple algorithm for displaying open reading frames longer than a specified number of residues in addition to plots of codon-specific G+C%, Fickett's testcode, Uneven Positional Base Frequencies, Positional Base Preference and three Codon Preference algorithms (MacVector, Staden and Gribskov) that plot coding probability using pre-calculated organism-specific codon usage tables. MacVector bundles a number of algorithms useful for identifying protein coding open reading frames into a single Coding Preference Toolbox. The output can be viewed graphically and also as a text view where the segments of similarity from the second sequence are displayed aligned underneath the annotated first sequence. MacVector lets you not only compare pairs of DNA sequences, but also a DNA sequence with a protein sequence. You can use Dot-plot comparisons to identify weak relationships between sequences or to identify repeat sequences. MacVector ships with a number of collections of interesting sites, including the popular tfsites Transcription Factor database (). However, you can use subsequences with complex patterns for the search - each pattern can have up to three distinct segments, separated by variable inter-segment regions, and you can control the overall similarity required for a match as well as defining residues which must be 100% conserved. MacVector can search for DNA subsequences using a very similar approach to the Restriction Enzyme searching functionality. You can also choose to display all of the enzymes that do not cut the selected DNA segment and lists of the fragments that would be produced by all combinations of single and double digests. The predicted cut sites can be listed in a variety of ways and displayed on an text-based annotated sequence as well as an interactive graphical map of the sequence. Once the scan has completed, you can apply additional post-processing filtering to help identify cut sites of interest and what information you would like to display. When you perform a search, you can not only choose the enzyme file you want to use, but you can also choose how you want the search to be performed - you can simply search using all enzymes in the file, or just the selected enzymes, or you can choose combinations of other criteria such as site size, end structure or number of cuts in the molecule. You can also select individual enzymes within each file to allow fine control over the enzymes used in a search. You can duplicate and/or edit these files to create your own custom lists of enzymes or even add new specificities. It maintains lists of enzymes in individual files representing not only all of the enzymes in the database, but also broken out my manufacturer. MacVector can search DNA sequences for restriction sites using any or all of the enzymes from the REBASE Restriction Enzyme Database ( ). You can also plot and list melting temperature statistics under a variety of experimental conditions. You can also plot base composition across a DNA segment, combine plots onto a single graph and zoom in to view regions of interest in more detail. You can list the total mono-, di- and tri-nucleotide frequencies within a selected segment of DNA and plot the occurrence of individual di- or tri-nucleotides (e.g. MacVector lets you plot and/or list a wide variety of base composition statistics, all driven from a single easy to use dialog. Sequence Analysis Tools for Molecular Biologists |
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